Name: TRYPSIN
Brand: Bioseutica

Trypsin grades

The proteolytic action of trypsin is used across broad contexts, from mass spectrometry to meat tenderising. In vaccine production, cells are detached from their vessels with trypsin, which cuts cell attachment proteins without harming the cells. In whey protein hydrolysis it makes amino acids more readily bioavailable.

The charts below show which grade suits which application, and the USP specifications of each grade. Select a grade to open its product page.

Applications per grade

Medical, pharmaceutical and research
Food processing, infant formula and dietary supplements

TRYPSIN VI Medical, pharmaceutical and research: 1Food processing, infant formula and dietary supplements: 8
TRYPSIN IV Medical, pharmaceutical and research: 2Food processing, infant formula and dietary supplements: 0
TRYPSIN II Medical, pharmaceutical and research: 2Food processing, infant formula and dietary supplements: 6
TRYPSIN I Medical, pharmaceutical and research: 4Food processing, infant formula and dietary supplements: 1
TRYPSIN 1:250 Medical, pharmaceutical and research: 2Food processing, infant formula and dietary supplements: 0
TRYPSIN SE Medical, pharmaceutical and research: 2Food processing, infant formula and dietary supplements: 6

Application suitability

A mark shows that a grade suits the application. Select a grade or a mark to open the product page.

Medical, pharmaceutical and research

Application	TRYPSIN VI	TRYPSIN IV	TRYPSIN II	TRYPSIN I	TRYPSIN 1:250	TRYPSIN SEPancreatin
Vaccine production	Not indicated	TRYPSIN IV suits Vaccine production	TRYPSIN II suits Vaccine production	TRYPSIN I suits Vaccine production	TRYPSIN 1:250 suits Vaccine production	Not indicated
Cell and tissue culture	Not indicated	TRYPSIN IV suits Cell and tissue culture	TRYPSIN II suits Cell and tissue culture	TRYPSIN I suits Cell and tissue culture	TRYPSIN 1:250 suits Cell and tissue culture	Not indicated
Mass spectrometry	Not indicated	Not indicated	Not indicated	TRYPSIN I suits Mass spectrometry	Not indicated	Not indicated
Diabetes and ophthalmic research	Not indicated	Not indicated	Not indicated	Not indicated	Not indicated	TRYPSIN SE suits Diabetes and ophthalmic research
USP / EP guideline conformance	Not indicated	Not indicated	Not indicated	TRYPSIN I suits USP / EP guideline conformance	Not indicated	Not indicated
Reduce oedema and bruising	TRYPSIN VI suits Reduce oedema and bruising	Not indicated	Not indicated	Not indicated	Not indicated	Not indicated
Enteral feeding	Not indicated	Not indicated	Not indicated	Not indicated	Not indicated	TRYPSIN SE suits Enteral feeding

Vaccine production TRYPSIN IV TRYPSIN II TRYPSIN I TRYPSIN 1:250
Cell and tissue culture TRYPSIN IV TRYPSIN II TRYPSIN I TRYPSIN 1:250
Mass spectrometry TRYPSIN I
Diabetes and ophthalmic research TRYPSIN SE
USP / EP guideline conformance TRYPSIN I
Reduce oedema and bruising TRYPSIN VI
Enteral feeding TRYPSIN SE

Food processing, infant formula and dietary supplements

Application	TRYPSIN VI	TRYPSIN IV	TRYPSIN II	TRYPSIN I	TRYPSIN 1:250	TRYPSIN SEPancreatin
Whey protein hydrolysis	TRYPSIN VI suits Whey protein hydrolysis	Not indicated	TRYPSIN II suits Whey protein hydrolysis	TRYPSIN I suits Whey protein hydrolysis	Not indicated	TRYPSIN SE suits Whey protein hydrolysis
Production of infant formula	TRYPSIN VI suits Production of infant formula	Not indicated	TRYPSIN II suits Production of infant formula	Not indicated	Not indicated	TRYPSIN SE suits Production of infant formula
Production of hypoallergenic infant formula	TRYPSIN VI suits Production of hypoallergenic infant formula	Not indicated	TRYPSIN II suits Production of hypoallergenic infant formula	Not indicated	Not indicated	TRYPSIN SE suits Production of hypoallergenic infant formula
Food processing	TRYPSIN VI suits Food processing	Not indicated	TRYPSIN II suits Food processing	Not indicated	Not indicated	TRYPSIN SE suits Food processing
Meat and seafood tenderisation	TRYPSIN VI suits Meat and seafood tenderisation	Not indicated	TRYPSIN II suits Meat and seafood tenderisation	Not indicated	Not indicated	Not indicated
Enhancement of food digestibility	TRYPSIN VI suits Enhancement of food digestibility	Not indicated	TRYPSIN II suits Enhancement of food digestibility	Not indicated	Not indicated	TRYPSIN SE suits Enhancement of food digestibility
Dietary supplementation	TRYPSIN VI suits Dietary supplementation	Not indicated	Not indicated	Not indicated	Not indicated	TRYPSIN SE suits Dietary supplementation
Functional foods	TRYPSIN VI suits Functional foods	Not indicated	Not indicated	Not indicated	Not indicated	Not indicated

Whey protein hydrolysis TRYPSIN VI TRYPSIN II TRYPSIN I TRYPSIN SE
Production of infant formula TRYPSIN VI TRYPSIN II TRYPSIN SE
Production of hypoallergenic infant formula TRYPSIN VI TRYPSIN II TRYPSIN SE
Food processing TRYPSIN VI TRYPSIN II TRYPSIN SE
Meat and seafood tenderisation TRYPSIN VI TRYPSIN II
Enhancement of food digestibility TRYPSIN VI TRYPSIN II TRYPSIN SE
Dietary supplementation TRYPSIN VI TRYPSIN SE
Functional foods TRYPSIN VI

The charts outline our experience of the optimal applications per trypsin grade. They are not exhaustive, but a starting point to select a grade for your use case.

USP specifications per grade

Minimum or maximum values per grade; “—” means not specified.

Specification	TRYPSIN VI	TRYPSIN IV	TRYPSIN II	TRYPSIN I	TRYPSIN 1:250	TRYPSIN SEPancreatin
Trypsin U/mg USP	≥ 2500	≥ 250	≥ 450	> 2500	≥ 250	—
Chymotrypsin U/mg USP	≥ 250	≤ 75	≤ 75	≤ 50	≥ 75	—
Endotoxin EU/mg	—	≤ 10	≤ 10	—	≤ 10	—
Protease U/mg USP	—	—	—	—	> 220	> 250

TRYPSIN VI

Trypsin U/mg USP
≥ 2500
Chymotrypsin U/mg USP
≥ 250
TRYPSIN IV

Trypsin U/mg USP
≥ 250
Chymotrypsin U/mg USP
≤ 75
Endotoxin EU/mg
≤ 10
TRYPSIN II

Trypsin U/mg USP
≥ 450
Chymotrypsin U/mg USP
≤ 75
Endotoxin EU/mg
≤ 10
TRYPSIN I

Trypsin U/mg USP
> 2500
Chymotrypsin U/mg USP
≤ 50
TRYPSIN 1:250

Trypsin U/mg USP
≥ 250
Chymotrypsin U/mg USP
≥ 75
Endotoxin EU/mg
≤ 10
Protease U/mg USP
> 220
TRYPSIN SE Pancreatin

Protease U/mg USP
> 250

Regulatory. Regulations may vary by country. Always check the local legislation on the use and claims of this product.

No warranty. The information is provided in good faith and, to the best of our knowledge, is true and correct. No warranty or guarantee is implied, and it may change without notice. Products are sold on the understanding that the purchaser tests their suitability for the intended use.